Ed.: 2 . Vig.: 09.09.2011 . |
| Prod.: 555229 |
КАТАЛОЖНЫЙ НОМЕР: 555229
HEPES Free Кислота (Buffers & Detergents) LS
(Высокий purity grade)
C8H18N2O4S
M.= 238,30 CAS [7365-45-9] EINECS 230-907-9
NC 2933 59 95
Синонимы: HEPES, N-2-Hydroxyethylpiperazin-N'-2-Ethanesulphonic Кислота•
ХАРАКТЕРИСТИКИ: порошок Белый • Растворяется в воде. M.P.: 238 - 240 °C • pEbu pH indrefraccionpKa20 °C7,55 • tolerancia
Применение:Biological буферн.: N.E. Good, et al.: Biochemistry 5 , 467 (1966); P.M. Brannon, et al., В vitro Cell. Dev. Biol. 21 , 6 (1985). One the best general purpose buffers available for biological research. Has been used до advantage в tissue culture, oxidative phosphorylation, protein synthesis с кювета-free bacterial systems, photophosphorylation, CO2 fixation; Suitable буферн. for TEM studies (Transmission Electron Microscopy) как it does not affect металл substrates; Recommended буферн. for the glutamate binding содержание, prevents binding до non-receptor materials.
Библиография:: Merck Index 12, 4.687 Beilstein 23 , 2 V , 379 • BRN 883043 •
Опасная Доза:
Спецификация:
| |
Минимальное содержание (Безводн. basis) | 99,0 % |
pka при 20 º C | 7,45 - 7,65 |
МАКСИМАЛЬНОЕ СОДЕРЖАНИЕ ПРИМЕСЕЙ |
|
Остаток при сжигании | 0,2 % |
Днказа | Не обнаружен |
Рнказа | Не обнаружен |
Протеаза | Не обнаружен |
Fe | 0,0005 % |
Тяжелые металлы (как Pb) | 0,0005 % |
|
Ed.: 2 . Vig.: 09.09.2011 . |
| Prod.: 555229 |
PRODUCT CODE: 555229
HEPES Free Acid (Buffers & Detergents) LS
(High purity grade)
C8H18N2O4S
M.= 238,30 CAS [7365-45-9] EINECS 230-907-9
NC 2933 59 95
SYNONYMS: HEPES, N-2-Hydroxyethylpiperazin-N'-2-Ethanesulphonic Acid•
PHYSICAL DATA: powder White • Soluble in water. M.P.: 238 - 240 °C • pEbu pH indrefraccionpKa20 °C7,55 • tolerancia
APPLICATIONS:Biological buffer: N.E. Good, et al.: Biochemistry 5 , 467 (1966); P.M. Brannon, et al., In vitro Cell. Dev. Biol. 21 , 6 (1985). One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials.
BIBLIOGRAPHY:: Merck Index 12, 4.687 Beilstein 23 , 2 V , 379 • BRN 883043 •
HAZARDOUS:
SPECIFICATIONS:
| |
Minimum assay (Dry basis) | 99,0 % |
pka at 20 º C | 7,45 - 7,65 |
MAXIMUM LIMIT OF IMPURITIES |
|
Residue on ignition | 0,2 % |
DNase | None detected |
RNase | None detected |
Protease | None detected |
Fe | 0,0005 % |
Heavy metals (as Pb) | 0,0005 % |
|